Category Archives: PCR

Successfully develop a PCR protocol to detect low copy DNA

Posted on September 19, 2012 by

I am interested in studying post-transcriptional regulatory mechanisms that occur during tumorigenesis. One of the major technical challenges of my project is being able to determine expression of exogenously introduced mutated genes in a sample. Developing a PCR assay to … Continue reading →

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Bioline Scholar: Focus on Autism Spectrum Disorders

Posted on September 18, 2012 by

Autism spectrum disorders (ASDs), or pervasive developmental disorders (PDDs), are a category of complex developmental brain disorders that appear in early childhood, usually before the age of three. ASDs cause difficulties in social, communication and behavioural skills, but affected individuals … Continue reading →

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Bioline Scholar: Focus on MRSA

Posted on September 18, 2012 by

The groundbreaking discovery by Sir Alexander Fleming of the first antibiotic penicillin in 1929, and its subsequent isolation by Ernst Chain and Sir Howard Florey in 1939, led to the award of the Nobel prize in 1945. This discovery revolutionized … Continue reading →

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Bioline Scholar: Plant Epigenetics

Posted on September 18, 2012 by

Focus on Plant Epigenetics 2012 marks the 150th anniversary of the publication of Charles Darwin’s first botanical book, on the fertilization of orchids (1862), wherein he described pollen grains and outlined his evolutionary principles with respect to plant research. Gregor … Continue reading →

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How to Take Your PCR From 11 Hours to TWO

Posted on March 14, 2012 by

We caught Fides Lay in the lab on the day of a basketball game between UCLA and crosstown arch-rival USC. As a former UCLA undergrad now working on her PhD at USC, she never wants to miss a game between … Continue reading →

Posted in Bisulfite, Cancer, Epigenetics, MyTaq, Newsletter, PCR | 1 Comment